LOCUS       hu DNA-PKcs      17053 bp    DNA     circular SYN 14-APR-2025
DEFINITION  human wild type DNA-PKcs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17053)
  AUTHORS   Cui X, Yu Y, Gupta S, Cho YM, Lees-Miller SP, Meek K
  TITLE     Autophosphorylation of DNA-dependent protein kinase regulates DNA 
            end processing and may also alter double-strand break repair pathway
            choice.
  JOURNAL   Mol Cell Biol. 2005 Dec;25(24):10842-52.
  PUBMED    16314509
REFERENCE   2  (bases 1 to 17053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            //gt12365.cn
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-12"; volume: "25(24)"; pages: "10842-52"
COMMENT     Sequence Label: hu DNA-PKcs
FEATURES             Location/Qualifiers
     source          1..17053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    13077..13699
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(13276..13295)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(13723..13743)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13728..14057
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13908..14043
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(14095..14114)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14096..14114)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14128..14144)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14128..14144)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14141..14163)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14152..14168
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14176..14182,14183..14200,14201..14206))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14221..14242
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14359..14376)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14530..15118)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14610..14629)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15289..16080,16081..16149))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15912..15931
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16150..16254)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      16281..16736
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16368..16387)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16578..16599
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     16862..16884
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     16876..16893
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16877..16893
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattcaga tctggtacca cgcgtatcga taagcttgca tgcctgcagg tcgactctag
      661 agcggccgca tggcgggctc cggagccggt gtgcgttgct ccctgctgcg gctgcaggag
      721 accttgtccg ctgcggaccg ctgcggtgct gccctggccg gtcatcaact gatccgcggc
      781 ctggggcagg aatgcgtcct gagcagcagc cccgcggtgc tggcattaca gacatcttta
      841 gttttttcca gagatttcgg tttgcttgta tttgtccgga agtcactcaa cagtattgaa
      901 tttcgtgaat gtagagaaga aatcctaaag tttttatgta ttttcttaga aaaaatgggc
      961 cagaagatcg caccttactc tgttgaaatt aagaacactt gtaccagtgt ttatacaaaa
     1021 gatagagctg ctaaatgtaa aattccagcc ctggaccttc ttattaagtt acttcagact
     1081 tttagaagtt ctagactcat ggatgaattt aaaattggag aattatttag taaattctat
     1141 ggagaacttg cattgaaaaa aaaaatacca gatacagttt tagaaaaagt atatgagctc
     1201 ctaggattat tgggtgaagt tcatcctagt gagatgataa ataatgcaga aaacctgttc
     1261 cgcgcttttc tgggtgaact taagacccag atgacatcag cagtaagaga gcccaaacta
     1321 cctgttctgg caggatgtct gaaggggttg tcctcacttc tgtgcaactt cactaagtcc
     1381 atggaagaag atccccagac ttcaagggag atttttaatt ttgtactaaa ggcaattcgt
     1441 cctcagattg atctgaagag atatgctgtg ccctcagctg gcttgcgcct atttgccctg
     1501 catgcatctc agtttagcac ctgccttctg gacaactacg tgtctctatt tgaagtcttg
     1561 ttaaagtggt gtgcccacac aaatgtagaa ttgaaaaaag ctgcactttc agccctggaa
     1621 tcctttctga aacaggtttc taatatggtg gcgaaaaatg cagaaatgca taaaaataaa
     1681 ctgcagtact ttatggagca gttttatgga atcatcagaa atgtggattc gaacaacaag
     1741 gagttatcta ttgctatccg tggatatgga ctttttgcag gaccgtgcaa ggttataaac
     1801 gcaaaagatg ttgacttcat gtacgttgag ctcattcagc gctgcaagca gatgttcctc
     1861 acccagacag acactggtga cgaccgtgtt tatcagatgc caagcttcct ccagtctgtt
     1921 gcaagcgtct tgctgtacct tgacacagtt cctgaggtgt atactccagt tctggagcac
     1981 ctcgtggtga tgcagataga cagtttccca cagtacagtc caaaaatgca gctggtgtgt
     2041 tgcagagcca tagtgaaggt gttcctagct ttggcagcaa aagggccagt tctcaggaat
     2101 tgcattagta ctgtggtgca tcagggttta atcagaatat gttctaaacc agtggtcctt
     2161 ccaaagggcc ctgagtctga atctgaagac caccgtgctt caggggaagt cagaactggc
     2221 aaatggaagg tgcccacata caaagactac gtggatctct tcagacatct cctgagctct
     2281 gaccagatga tggattctat tttagcagat gaagcatttt tctctgtgaa ttcctccagt
     2341 gaaagtctga atcatttact ttatgatgaa tttgtaaaat ccgttttgaa gattgttgag
     2401 aaattggatc ttacacttga aatacagact gttggggaac aagagaatgg agatgaggcg
     2461 cctggtgttt ggatgatccc aacttcagat ccagcggcta acttgcatcc agctaaacct
     2521 aaagattttt cggctttcat taacctggtg gaattttgca gagagattct ccctgagaaa
     2581 caagcagaat tttttgaacc atgggtgtac tcattttcat atgaattaat tttgcaatct
     2641 acaaggttgc ccctcatcag tggtttctac aaattgcttt ctattacagt aagaaatgcc
     2701 aagaaaataa aatatttcga gggagttagt ccaaagagtc tgaaacactc tcctgaagac
     2761 ccagaaaagt attcttgctt tgctttattt gtgaaatttg gcaaagaggt ggcagttaaa
     2821 atgaagcagt acaaagatga acttttggcc tcttgtttga cctttcttct gtccttgcca
     2881 cacaacatca ttgaactcga tgttagagcc tacgttcctg cactgcagat ggctttcaaa
     2941 ctgggcctga gctatacccc cttggcagaa gtaggcctga atgctctaga agaatggtca
     3001 atttatattg acagacatgt aatgcagcct tattacaaag acattctccc ctgcctggat
     3061 ggatacctga agacttcagc cttgtcagat gagaccaaga ataactggga agtgtcagct
     3121 ctttctcggg ctgcccagaa aggatttaat aaagtggtgt taaagcatct gaagaagaca
     3181 aagaaccttt catcaaacga agcaatatcc ttagaagaaa taagaattag agtagtacaa
     3241 atgcttggat ctctaggagg acaaataaac aaaaatcttc tgacagtcac gtcctcagat
     3301 gagatgatga agagctatgt ggcctgggac agagagaagc ggctgagctt tgcagtgccc
     3361 tttagagaga tgaaacctgt cattttcctg gatgtgttcc tgcctcgagt cacagaatta
     3421 gcgctcacag ccagtgacag acaaactaag gttgcagcct gtgaactttt acatagcatg
     3481 gttatgttta tgttgggcaa agccacgcag atgccagaag ggggacaggg agccccaccc
     3541 atgtaccagc tctataagcg gacgtttcct gtgctgcttc gacttgcgtg tgatgttgat
     3601 caggtgacaa ggcaactgta tgagccacta gttatgcagc tgattcactg gttcactaac
     3661 aacaagaaat ttgaaagtca ggatactgtt gccttactag aagctatatt ggatggaatt
     3721 gtggaccctg ttgacagtac tttaagagat ttttgtggtc ggtgtattcg agaattcctt
     3781 aaatggtcca ttaagcaaat aacaccacag cagcaggaga agagtccagt aaacaccaaa
     3841 tcgcttttca agcgacttta tagccttgcg cttcacccca atgctttcaa gaggctggga
     3901 gcatcacttg cctttaataa tatctacagg gaattcaggg aagaagagtc tctggtggaa
     3961 cagtttgtgt ttgaagcctt ggtgatatac atggagagtc tggccttagc acatgcagat
     4021 gagaagtcct taggtacaat tcaacagtgt tgtgatgcca ttgatcacct atgccgcatc
     4081 attgaaaaga agcatgtttc tttaaataaa gcaaagaaac gacgtttgcc gcgaggattt
     4141 ccaccttccg catcattgtg tttattggat ctggtcaagt ggcttttagc tcattgtggg
     4201 aggccccaga cagaatgtcg acacaaatcc attgaactct tttataaatt cgttccttta
     4261 ttgccaggca acagatcccc taatttgtgg ctgaaagatg ttctcaagga agaaggtgtc
     4321 tcttttctca tcaacacctt tgaggggggt ggctgtggcc agccctcggg catcctggcc
     4381 cagcccaccc tcttgtacct tcgggggcca ttcagcctgc aggccacgct atgctggctg
     4441 gacctgctcc tggccgcgtt ggagtgctac aacacgttca ttggcgagag aactgtagga
     4501 gcgctccagg tcctaggtac tgaagcccag tcttcacttt tgaaagcagt ggctttcttc
     4561 ttagaaagca ttgccatgca tgacattata gcagcagaaa agtgctttgg cactggggca
     4621 gcaggtaaca gaacaagccc acaagaggga gaaaggtaca actacagcaa atgcaccgtt
     4681 gtggtccgga ttatggagtt taccacgact ctgctaaaca cctccccgga aggatggaag
     4741 ctcctgaaga aggacttgtg taatacacac ctgatgagag tcctggtgca gacgctgtgt
     4801 gagcccgcaa gcataggttt caacatcgga gacgtccagg ttatggctca tcttcctgat
     4861 gtttgtgtga atctgatgaa agctctaaag atgtccccat acaaagatat cctagagacc
     4921 catctgagag agaaaataac agcacagagc attgaggagc tttgtgccgt caacttgtat
     4981 ggccctgacg cgcaagtgga caggagcagg ctggctgctg ttgtgtctgc ctgtaaacag
     5041 cttcacagag ctgggcttct gcataatata ttaccgtctc agtccacaga tttgcatcat
     5101 tctgttggca cagaacttct ttccctggtt tataaaggca ttgcccctgg agatgagaga
     5161 cagtgtctgc cttctctaga cctcagttgt aagcagctgg ccagcggact tctggagtta
     5221 gcctttgctt ttggaggact gtgtgagcgc cttgtgagtc ttctcctgaa cccagcggtg
     5281 ctgtccacgg cgtccttggg cagctcacag ggcagcgtca tccacttctc ccatggggag
     5341 tatttctata gcttgttctc agaaacgatc aacacggaat tattgaaaaa tctggatctt
     5401 gctgtattgg agctcatgca gtcttcagtg gataatacca aaatggtgag tgccgttttg
     5461 aacggcatgt tagaccagag cttcagggag cgagcaaacc agaaacacca aggactgaaa
     5521 cttgcgacta caattctgca acactggaag aagtgtgatt catggtgggc caaagattcc
     5581 cctctcgaaa ctaaaatggc agtgctggcc ttactggcaa aaattttaca gattgattca
     5641 tctgtatctt ttaatacaag tcatggttca ttccctgaag tctttacaac atatattagt
     5701 ctacttgctg acacaaagct ggatctacat ttaaagggcc aagctgtcac tcttcttcca
     5761 ttcttcacca gcctcactgg aggcagtctg gaggaactta gacgtgttct ggagcagctc
     5821 atcgttgctc acttccccat gcagtccagg gaatttcctc caggaactcc gcggttcaat
     5881 aattatgtgg actgcatgaa aaagtttcta gatgcattgg aattatctca aagccctatg
     5941 ttgttggaat tgatgacaga agttctttgt cgggaacagc agcatgtcat ggaagaatta
     6001 tttcaatcca gtttcaggag gattgccaga aggggttcat gtgtcacaca agtaggcctt
     6061 ctggaaagcg tgtatgaaat gttcaggaag gatgaccccc gcctaagttt cacacgccag
     6121 tcctttgtgg accgctccct cctcactctg ctgtggcact gtagcctgga tgctttgaga
     6181 gaattcttca gcacaattgt ggtggatgcc attgatgtgt tgaagtccag gtttacaaag
     6241 ctaaatgaat ctacctttga tactcaaatc accaagaaga tgggctacta taagattcta
     6301 gacgtgatgt attctcgcct tcccaaagat gatgttcatg ctaaggaatc aaaaattaat
     6361 caagttttcc atggctcgtg tattacagaa ggaaatgaac ttacaaagac attgattaaa
     6421 ttgtgctacg atgcatttac agagaacatg gcaggagaga atcagctgct ggagaggaga
     6481 agactttacc attgtgcagc atacaactgc gccatatctg tcatctgctg tgtcttcaat
     6541 gagttaaaat tttaccaagg ttttctgttt agtgaaaaac cagaaaagaa cttgcttatt
     6601 tttgaaaatc tgatcgacct gaagcgccgc tataattttc ctgtagaagt tgaggttcct
     6661 atggaaagaa agaaaaagta cattgaaatt aggaaagaag ccagagaagc agcaaatggg
     6721 gattcagatg gtccttccta tatgtcttcc ctgtcatatt tggcagacag taccctgagt
     6781 gaggaaatga gtcaatttga tttctcaacc ggagttcaga gctattcata cagctcccaa
     6841 gaccctagac ctgccactgg tcgttttcgg agacgggagc agcgggaccc cacggtgcat
     6901 gatgatgtgc tggagctgga gatggacgag ctcaatcggc atgagtgcat ggcgcccctg
     6961 acggccctgg tcaagcacat gcacagaagc ctgggcccgc ctcaaggaga agaggattca
     7021 gtgccaagag atcttccttc ttggatgaaa ttcctccatg gcaaactggg aaatccaata
     7081 gtaccattaa atatccgtct cttcttagcc aagcttgtta ttaatacaga agaggtcttt
     7141 cgcccttacg cgaagcactg gcttagcccc ttgctgcagc tggctgcttc tgaaaacaat
     7201 ggaggagaag gaattcacta catggtggtt gagatagtgg ccactattct ttcatggaca
     7261 ggcttggcca ctccaacagg ggtccctaaa gatgaagtgt tagcaaatcg attgcttaat
     7321 ttcctaatga aacatgtctt tcatccaaaa agagctgtgt ttagacacaa ccttgaaatt
     7381 ataaagaccc ttgtcgagtg ctggaaggat tgtttatcca tcccttatag gttaatattt
     7441 gaaaagtttt ccggtaaaga tcctaattct aaagacaact cagtagggat tcaattgcta
     7501 ggcatcgtga tggccaatga cctgcctccc tatgacccac agtgtggcat ccagagtagc
     7561 gaatacttcc aggctttggt gaataatatg tcctttgtaa gatataaaga agtgtatgcc
     7621 gctgcagcag aagttctagg acttatactt cgatatgtta tagagagaaa aaacatactg
     7681 gaggagtctc tgtgtgaact ggttgcgaaa caattgaagc aacatcagaa tactatggag
     7741 gacaagttta ttgtgtgctt gaacaaagtg accaagagct tccctcctct tgcagacagg
     7801 ttcatgaatg ctgtgttctt tctgctgcca aaatttcatg gagtgttgaa aacactctgt
     7861 ctggaggtgg tactttgtcg tgtggaggga atgacagagc tgtacttcca gttaaagagc
     7921 aaggacttcg ttcaagtcat gagacataga gatgatgaaa gacaaaaagt atgtttggac
     7981 ataatttata agatgatgcc aaagttaaaa ccagtagaac tccgagaact tctgaacccc
     8041 gttgtggaat tcgtttccca tccttctaca acatgtaggg aacaaatgta taatattctc
     8101 atgtggattc atgataatta cagagatcca gaaagtgaga cagataatga ctcccaggaa
     8161 atatttaagt tggcaaaaga tgtgctgatt caaggattga tcgatgagaa ccctggactt
     8221 caattaatta ttcgaaattt ctggagccat gaaactaggt taccttcaaa taccttggac
     8281 cggttgctgg cactaaattc cttatattct cctaagatag aagtgcactt tttaagttta
     8341 gcaacaaatt ttctgctcga aatgaccagc atgagcccag attatccaaa ccccatgttc
     8401 gagcatcctc tgtcagaatg cgaatttcag gaatatacca ttgattctga ttggcgtttc
     8461 cgaagtactg ttctcactcc gatgtttgtg gagacccagg cctcccaggg cactctccag
     8521 gcccgtaccc aggaagggtc cctctcagct cgctggccag tggcagggca gataagggcc
     8581 acccagcagc agcatgactt cacactgaca cagactgcag atggaagaag ctcatttgat
     8641 tggctgaccg ggagcagcac tgacccgctg gtcgaccaca ccagtccctc atctgactcc
     8701 ttgctgtttg cccacaagag gagtgaaagg ttacagagag cacccttgaa gtcagtgggg
     8761 cctgattttg ggaaaaaaag gctgggcctt ccaggggacg aggtggataa caaagtgaaa
     8821 ggtgcggccg gccggacgga cctactacga ctgcgcagac ggtttatgag ggaccaggag
     8881 aagctcagtt tgatgtatgc cagaaaaggc gttgctgagc aaaaacgaga gaaggaaatc
     8941 aagagtgagt taaaaatgaa gcaggatgcc caggtcgttc tgtacagaag ctaccggcac
     9001 ggagaccttc ctgacattca gatcaagcac agcagcctca tcaccccgtt acaggccgtg
     9061 gcccagaggg acccaataat tgcaaaacag ctctttagca gcttgttttc tggaattttg
     9121 aaagagatgg ataaatttaa gacactgtct gaaaaaaaca acatcactca aaagttgctt
     9181 caagacttca atcgttttct taataccacc ttctctttct ttccaccctt tgtctcttgt
     9241 attcaggaca ttagctgtca gcacgcagcc ctgctgagcc tcgacccagc ggctgttagc
     9301 gctggttgcc tggccagcct acagcagccc gtgggcatcc gcctgctaga ggaggctctg
     9361 ctctgcctgc tgcctgctga gctgcccgcc aagcgagtcc gtgggaaggc ccgcccccct
     9421 cctgatgtcc tcagatgggt ggagcttgct aagctgtata gatcaattgg agaatacgac
     9481 gtcctccgtg ggatttttac cagtgagata ggaacaaagc aaatcactca gagtgcatta
     9541 ttagcagaag ccagaagtga ttattctgaa gctgctaagc agtatgatga ggctctcaat
     9601 aaacaagact gggtagatgg tgagcccaca gaagccgaga aggatttttg ggaacttgca
     9661 tcccttgact gttacaacca ccttgctgag tggaaatcac ttgaatactg ttctacagcc
     9721 agtatagaca gtgagaaccc cccagaccta aataaaatct ggagtgaacc attttatcag
     9781 gaaacatatc taccttacat gatccgcagc aagctgaagc tgctgctcca gggagaggct
     9841 gaccagtccc tgctgacatt tattgacaaa gctatgcacg gggagctcca gaaggcgatt
     9901 ctagagcttc attacagtca agagctgagt ctgctttacc tcctgcaaga tgatgttgac
     9961 agagccaaat attacattca aaatggcatt cagagtttta tgcagaatta ttctagtatt
    10021 gatgtcctct tacaccaaag tagactcacc aaattgcagt ctgtacaggc tttaacagaa
    10081 attcaggagt tcatcagctt tataagcaaa caaggcaatt tatcatctca agttcccctt
    10141 aagagacttc tgaacacctg ggcaaacaga tatccagatg ctaaaatgga cccaatgaac
    10201 atctgggatg acatcatcac aaatcgatgt ttctttctca gcaaaataga ggagaagctt
    10261 acccctcttc cagaagataa tagtatgaat gtggatcaag atggagaccc cagtgacagg
    10321 atggaagtgc aagagcagga agaagatatc agctccctga tcaggagttg caagttttcc
    10381 atgaaaatga agatgataga cagtgcccgg aagcagaaca atttctcact tgctatgaaa
    10441 ctactgaagg agctgcataa agagtcaaaa accagagacg attggctggt gagctgggtg
    10501 cagagctact gccgcctgag ccactgccgg agccggtccc agggctgctc tgagcaggtg
    10561 ctcactgtgc tgaaaacagt ctctttgttg gatgagaaca acgtgtcaag ctacttaagc
    10621 aaaaatattc tggctttccg tgaccagaac attctcttgg gtacaactta caggatcata
    10681 gcgaatgctc tcagcagtga gccagcctgc cttgctgaaa tcgaggagga caaggctaga
    10741 agaatcttag agctttctgg atccagttca gaggattcag agaaggtgat cgcgggtctg
    10801 taccagagag cattccagca cctctctgag gctgtgcagg cggctgagga ggaggcccag
    10861 cctccctcct ggagctgtgg gcctgcagct ggggtgattg atgcttacat gacgctggca
    10921 gatttctgtg accaacagct gcgcaaggag gaagagaatg catcagttac tgattctgca
    10981 gaactgcagg cgtatccagc acttgtggtg gagaaaatgt tgaaagcttt aaaattaaat
    11041 tccaatgaag ccagattgaa gtttcctaga ttacttcaga ttatagaacg gtatccagag
    11101 gagactttga gcctcatgac aaaagagatc tcttccgttc cctgctggca gttcatcagc
    11161 tggatcagcc acatggtggc cttactggac aaagaccaag ccgttgctgt tcagcactct
    11221 gtggaagaaa tcactgataa ctacccgcag gctattgttt atcccttcat cataagcagc
    11281 gaaagctatt ccttcaagga tacttctact ggtcataaga ataaggagtt tgtggcaagg
    11341 attaaaagta agttggatca aggaggagtg attcaagatt ttattaatgc cttagatcag
    11401 ctctctaatc ctgaactgct ctttaaggat tggagcaatg atgtaagagc tgaactagca
    11461 aaaacccctg taaataaaaa aaacattgaa aaaatgtatg aaagaatgta tgcagccttg
    11521 ggtgacccaa aggctccagg cctgggggcc tttagaagga agtttattca gacttttgga
    11581 aaagaatttg ataaacattt tgggaaagga ggttctaaac tactgagaat gaagctcagt
    11641 gacttcaacg acattaccaa catgctactt ttaaaaatga acaaagactc aaagccccct
    11701 gggaatctga aagaatgttc accctggatg agcgacttca aagtggagtt cctgagaaat
    11761 gagctggaga ttcccggtca gtatgacggt aggggaaagc cattgccaga gtaccacgtg
    11821 cgaatcgccg ggtttgatga gcgggtgaca gtcatggcgt ctctgcgaag gcccaagcgc
    11881 atcatcatcc gtggccatga cgagagggaa caccctttcc tggtgaaggg tggcgaggac
    11941 ctgcggcagg accagcgcgt ggagcagctc ttccaggtca tgaatgggat cctggcccaa
    12001 gactccgcct gcagccagag ggccctgcag ctgaggacct atagcgttgt gcccatgacc
    12061 tccaggttag gattaattga gtggcttgaa aatactgtta ccttgaagga ccttcttttg
    12121 aacaccatgt cccaagagga gaaggcggct tacctgagtg atcccagggc accgccgtgt
    12181 gaatataaag attggctgac aaaaatgtca ggaaaacatg atgttggagc ttacatgcta
    12241 atgtataagg gcgctaatcg tactgaaaca gtcacgtctt ttagaaaacg agaaagtaaa
    12301 gtgcctgctg atctcttaaa gcgggccttc gtgaggatga gtacaagccc tgaggctttc
    12361 ctggcgctcc gctcccactt cgccagctct cacgctctga tatgcatcag ccactggatc
    12421 ctcgggattg gagacagaca tctgaacaac tttatggtgg ccatggagac tggtggcgtg
    12481 atcgggatcg actttgggca tgcgtttgga tccgctacac agtttctgcc agtccctgag
    12541 ttgatgcctt ttcggctaac tcgccagttt atcaatctga tgttaccaat gaaagaaacg
    12601 ggccttatgt acagcatcat ggtacacgca ctccgggcct tccgctcaga ccctggcctg
    12661 ctcaccaaca ccatggatgt gtttgtcaag gagccctcct ttgattggaa aaattttgaa
    12721 cagaaaatgc tgaaaaaagg agggtcatgg attcaagaaa taaatgttgc tgaaaaaaat
    12781 tggtaccccc gacagaaaat atgttacgct aagagaaagt tagcaggtgc caatccagca
    12841 gtcattactt gtgatgagct actcctgggt catgagaagg cccctgcctt cagagactat
    12901 gtggctgtgg cacgaggaag caaagatcac aacattcgtg cccaagaacc agagagtggg
    12961 ctttcagaag agactcaagt gaagtgcctg atggaccagg caacagaccc caacatcctt
    13021 ggcagaacct gggaaggatg ggagccctgg atgtgaggtc tgcggccgcg gatcccgggt
    13081 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc
    13141 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct
    13201 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct
    13261 gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca
    13321 ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg
    13381 gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt
    13441 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc
    13501 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt
    13561 taaaataact ataccagcag gaggacgtcc agacacagca taggctacct ggccatgccc
    13621 aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt gggtccactc
    13681 agtagatgcc tgttgaattg ggtacgcggc cagcttggct gtggaatgtg tgtcagttag
    13741 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    13801 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    13861 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    13921 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    13981 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    14041 gcctaggctt ttgcaaaaag ctcctcgagg aactgaaaaa ccagaaagtt aattccctat
    14101 agtgagtcgt attaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
    14161 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    14221 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    14281 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    14341 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    14401 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    14461 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    14521 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    14581 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    14641 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    14701 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    14761 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    14821 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    14881 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    14941 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    15001 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    15061 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    15121 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    15181 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    15241 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    15301 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    15361 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    15421 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    15481 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    15541 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    15601 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    15661 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    15721 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    15781 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    15841 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    15901 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    15961 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    16021 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    16081 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    16141 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    16201 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    16261 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    16321 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    16381 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    16441 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    16501 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    16561 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    16621 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    16681 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat
    16741 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    16801 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    16861 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tgatctatac attgaatcaa
    16921 tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg
    16981 ccattgcata cgttgtatct atatcataat atgtacattt atattggctc atgtccaata
    17041 tgaccgccat gtt
//